E-IP Test™ cDNA Synthesis with Oligo(dT) Primers Kit includes three components that have been optimized to use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.
The Reverse Transcriptase Mix (contains Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water. This kit can be used to reverse transcribe total RNA, mRNA, or synthetic transcript RNA, and is required for gene expression analyses such as qPCR and RNA sequencing.
This Kit is available with either oligo(dT) or random primers. For random primers please contact us on Info@cellulim.com.
E-IP Test™ cDNA Synthesis with Oligo(dT) Primers