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Whole genome sequencing


Are you looking to unlock the secrets of your DNA? Whole genome sequencing is the most comprehensive way to understand your genetic makeup and learn about the genetic basis of your traits and characteristics.

With whole genome sequencing, you can:

  • Understand your risk for certain diseases and conditions, and take proactive steps to protect your health
  • Identify genetic variations that may be used to personalize your medical treatment
  • Learn about your ancestry and family history
  • Contribute to scientific research and our understanding of genetics

Don't miss this opportunity to get a deep understanding of your DNA and learn more about your unique genetic profile. Order your whole genome sequencing test today!

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Category: screening tests

Give your self a change to discover who you really are.

With whole-genome sequencing (WGS) you have a comprehensive method for analyzing your entire genomes. With your genomic information at hand you have the key to access imaginable information about your self.

We each have our unique genetic code. And if you’ve tapped into that code: you know the value of learning more about who you are and where you came from.

The beauty of whole DNA sequencing is that your raw sequenced data hold so much information that can be tapped immediately but also in the future when more and more DNA insights are discovered.

When you have access to your raw DNA data, you can unlock personal information about yourself trough our anti aging analysis reports or by simply uploading your file to other trusted DNA upload sites.

The information you can find is limitless. So what type of information do you want to know about yourself?

-  Identify inherited disorders

-  Characterize mutations that drive cancer progression

-  Find you family tree

-  Information about your nutrition And fitness

-   And many more..

The biological information retrieved from your whole genome sequencing gives you real insight on how to create an effective program for increasing your health span and change your life.

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To get the whole picture, sequence the whole genome

But how does it work, in general, genomic DNA is first randomly fragmented using sonication or nebulization, and then are ligated to a platform-specific set of double-stranded adapters to generate a shotgun library. Subsequently, these library fragments can be amplified in situ  by hybridization and extension from complementary adapters which are covalently attached to the surface of a glass microfluidic cell or a small bead (depending on the sequencing platform). All NGS instruments utilize a microfluidic device to contain the amplified fragments of the shotgun library, followed by an imaging step that collects data from fragments being actively sequenced.

Steps Workflow
Construction of Sequencing Library
The genome is first prepared, and then the DNA is randomly fragmented into hundreds of bases or shorter fragments with specific adapters at both ends. If the transcriptional group is sequenced, the library construction is a bit more troublesome. After the RNA fragmentation, it needs to reverse to cDNA, then add the connector, or reverse the RNA to the cDNA first, then fragment and add the joint. The size of the fragment (insert size) has an impact on the subsequent data analysis and can be selected according to needs. For genome sequencing, several different insert sizes are usually chosen to get more information when assembling.
Surface Attachment and Bridge Amplification
The reaction of Solexa sequencing is carried out in a glass tube called flow cell, and flow cell is subdivided into 8 Lanes, each of which has a number of fixed single strand joints on the inner surface of each Lane. The DNA fragment of the joint was transformed into a single strand and combined with the primers on the sequencing channel to form a bridge like structure for subsequent preamplification.
Denaturation and Complete Amplification
The unlabeled dNTP and the common Taq enzyme were added for solid phase bridge PCR amplification, and the single-stranded bridge sample was amplified into a double-stranded bridge fragment. By denaturation, a complementary single strand is released and anchored to the nearby solid surface. By continuously cycling, millions of clusters of double-stranded analytes will be obtained on the solid surface of the Flow cell.
Single Base Extension and Sequencing
Four fluorescently labeled dNTPs, DNA polymerases, and linker primers were added to the sequenced flow cells for amplification. When each sequencing cluster extends the complementary strand, each fluorescent labeled dNTP is added to release the corresponding fluorescence. The sequencer obtains sequence information of the fragment to be tested by capturing a fluorescent signal and converting the optical signal into a sequencing peak by computer software. The read length is affected by a number of factors that cause signal attenuation, such as incomplete cutting of fluorescent markers. As the length of the reading increases, the error rate will also increase.
Data Analysis
This step is not strictly a part of the sequencing process, but it only makes sense through the work in front of this step. The raw data obtained by sequencing is a sequence of only a few tens of bases in length, and the contigs that assemble these short sequences through bioinformatics tools are even the framework of the entire genome. Alternatively, these sequences are aligned to an existing genome or a similar species genome sequence, and further analyzed to obtain biologically meaningful results.

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